Phis1525
Webbb-Xylosidase encoding gene from G. thermoleovorans IT-08 had been expressed in the pHIS1525/ B. megaterium MS941 system. The b-xylosidase gene (xyl) was inserted into … WebbDownload dengan 2 jenis enzim restriksi SacI dan SphI, (2) rekombinasi. (ligasi) amplikon dan plasmid pHIS1525 menggunakan enzim ligase (3) transformasi plasmid ...
Phis1525
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WebbpHIS1525 Description: Plasmid type: Expression vector of Bacillus megaterium Size: 7402 bp Tags: 6xHis Resistance(s): Ampicillin (Amp) Selectable markers: Tetracycline (Tet) WebbTools. Data Hub. Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. Basic analysis for a user …
WebbThe pHIS1525 system is also equipped with a 6x His-tag sequence for purification and detection of the expressed His-tagged target proteins. 2, 3 In this work, we reported the … Webb24 sep. 2012 · 巨大芽孢杆菌表达外源蛋白的特点及其研究进展.pdf.pdf. 中国生物工程杂志Chinaiotechnology,2008,28 (93~97巨大芽孢杆菌表达外源蛋白的特点及其研究进展1四川 …
WebbEkspresi gen dalam sistem pHIS1525/B. megterium -xilosidase ekstraseluler. Tujuan penelitian ini adalah untuk -xilosidase asal IT-08 ke dalam plasmid pHIS1525 dan … Webbwith the CPD, by ligation into pHis1525/TcdB digested with 5-BsrGI and 3-BamHI. To generate AGT-TcdB-BoNT/A-Hc, an AgeI site was installed between the TD and RBD of TcdB. The entire RBD of TcdB, consisting of the C-terminally combined repetitive oligopeptides (CROPs, residues 1852–2366), was
WebbAbstract: Bacterial transformation is commonly used both in commercial applications and research laboratories to transfer one or more genes into a cell.
Webb4 dec. 2009 · ISBN: 978-981-4322-10-2 (hardcover) USD 199.00. ISBN: 978-981-4464-19-2 (ebook) USD 80.00. Description. Chapters. This book aims to disseminate the most current research in applied microbiology presented at the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009) held in Lisbon, … how do you lose lower belly fatWebb巨大芽孢杆菌低温保存法：1.简单保存法，将琼脂斜面孢子培养物、菌丝悬浮液以及由麸皮、大米、小米等谷物原料制成的孢子培养物置于4℃冰箱保存,保存时间不超过1～2个月，若将谷物原料制备的孢子瓶抽真空并在棉塞上浸蜡,以隔绝外界空气和水汽,保持时间可达3～4个 … phone cases + shopifyWebbEkspresi gen penyandi β-xilosidase dalam sistem pHIS1525/ Bacillus megaterium MS941 by: Sri Sumarsih, et al. Published: (2012-02-01) Implication of Polyhistidine, a Novel … phone cases 4WebbBoca Scientific vector phis1525 Vector Phis1525, supplied by Boca Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … how do you lose us citizenshipWebbFor secretion of fusion proteins, the open reading frames were cloned into the Escherichia coli-Bacillus megaterium shuttle vector pHIS1525. After transformation of the respective … how do you lose in chessWebbVisit ChemicalBook To find more pHIS1525() information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular … phone cases amazon huaweiWebbvector pHis1525 19. FliCD was expressed in B. megaterium and puried as described previously 20. Preparation of C. difficile spores Sporulation of the C. difficile R20291 strains were induced in Clospore medium as described previously 21. Briefl y, an overnight 20 ml of C. difficile cultured in Columbia Broth was inoculated into 500 ml of how do you lose water weight