WebRecommended gel percentage range: 1-1.4%; Optimum separation on 1.2% . 1 kb Plus DNA Ladder for Safe Stains visualized on a 1.0% TBE agarose gel precast with GelRed. Mass values are for 0.5 µg/lane. Suggested Load: 5 to 10 µl/lane. This product is related to the following categories: DNA Markers & Ladders Products WebIn this western blot troubleshooting section, we will help you visually identify specific and common problems on your western blots, such as high background, weak or no signal, multiple bands, uneven staining and suggest what may be causing them and some solutions to remedy them. Request a free Western blot tips, tricks and troubleshooting guide.
PCR Troubleshooting Bio-Rad
WebColumn-based methods shoud be work well for DNA purification tasks. But sometimes from many reasons (high pH (7.5<) at DNA binding step, or improper ethanol removal after wash step) the DNA... WebTroubleshooting your PCR What should you do when your PCR goes wrong? The FAQs below can set you on a path toward successful PCR. For tips on how to rescue your experiments from PCR contamination, check out this blog article. Expand All If no amplification products are obtained, what parameters should be considered first when … dynamic microphone cartridge slides around
Pre-Cast Denaturing Gels for High Resolution Nucleic Acid …
WebEffective Range of Separation of DNA in Polyacrylamide Gels; Effective Range of Separation of DNA in Agarose Gels; DNA Size Migration of Sample Loading Dyes; … WebA couple simple ways to increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer period of time; b) using a wider gel comb; … WebTroubleshooting Guide to Sanger Sequencing Good Sequencing The sequencing electropherogram will show machine model and analysis software version in the left hand corner. The sample name and investigator will appear to the middle left. On the middle right you will see notation showing signal strength. dynamic microphone cartridge response